Hi George. I'm far from an expert, but that's never stopped me before. Here's what I can make of the numbers you posted:
Your DC current through R43 looks to be about 16mA with tubes installed ((416V - 408V) / 500R = 16mA), and about 6mA without tubes ((448V - 445V) / 500R = 6mA). For comparison, the callouts on the schematic suggest about 8mA DC with tubes. Your R43 currents seem high.
You also have about (408V - 400V) / 1k = 8mA DC through R44 with tubes, and (445V - 443V) / 1k = 2mA without. The schematic calls for a 7V drop across R44 (i.e., 7mA through R44, with tubes). Without tubes, it looks like the only thing drawing current from B+2, B+3, B+4, B+5 should be R47. This works out to a little over 2mA DC through R43 and R44 without tubes. In other words, your R44 currents look about right.
So your B+2 current is about (16mA - 8mA) = 8mA with tubes, when we'd expect something closer to one or two mA.
And your B+2 current is about (6mA - 2mA) = 4mA without tubes. Without tubes, I don't think anything should be drawing current from B+2.
I'd look super-carefully for anything that might be drawing B+2 current. If you can't find anything, then maybe C32 is leaky? To check that, you could try disconnecting C32, powering up with no tubes, and seeing if your current through R43 drops down to ~2mA. Once that was sorted out, and the no-tubes voltages were looking more sane, I'd install tubes and double-check the bias on V4 and V5.
Disclaimer: this advice is worth what you paid for it.