Using the voltage chart ( 2 charts, one for 6V6, one for 6L6) in build guide and layout sheet, check AC voltages where the the 2 red wires from XFMR attach to the 6 diodes (the diodes rectify AC to DC) you can test with both probes on AC setting OR ground to chassis and + probe, but you'll only get 1/2 the AC voltage, the single red wire at the other end of the diodes should be measured using DC setting on your DMM (ground to chassis and red probe to point being tested)and all other voltages (B+ and various tube pins). Also, the little square boxes by the various tubes and eyelets in the layout indicate DC voltage you should see at those points. Short answer (usually) before the diodes AC, after DC. Hope this is somewhat clearer than mud.
